16S rRNA analysis of Listeria monocytogenes and Brochothrix thermosphacta

Reductions of Listeria innocua and Brochothrix thermosphacta on beef following nisin spray treatments and vacuum packaging

Sections of UV sterilized lean and adipose tissues from the surfaces of post-rigor (24 h post-mortem) beef carcasses were inoculated with Brochothrix thermosphacta or Listeria innocua to obtain approximately 4·50 log10 cfu cm and subjected to spray treatments with sterile water or nisin (5000 AU ml). Untreated and spray treated samples were vacuumpackaged, and incubated at 4°C for up to 4 weeks...

In situ detection of a virulence factor mRNA and 16S rRNA in Listeria monocytogenes.

Simultaneous in situ analysis of the structure and function of bacterial cells present within complex communities is a key for improving our understanding of microbial ecology. A protocol for the in situ identification of Listeria spp. using fluorescently tagged, rRNA-targeted oligonucleotide probes was developed. Ethanol fixation and enzymatic pretreatment with lysozyme and proteinase K were u...

End Products of Glucose Fermentation by Brochothrix thermosphacta.

Anaerobically, Brochothrix thermosphacta fermented glucose primarily to l-lactate, acetate, formate, and ethanol. The ratio of these end products varied with growth conditions. Both the presence of acetate and formate and a pH below about 6 increased l-lactate production from glucose. Small amounts of butane-2,3-diol were also produced when the pH of the culture was low (</=5.5) or when acetate...

Novel Approaches for the Efficient Sampling and Detection of <em>Listeria monocytogenes</em> and <em>Brochothrix thermosphacta</em> on Food Contact Surfaces

Comparative analysis of 16S and 23S rRNA sequences of Listeria species.

In order to establish the taxonomic value of 16S rRNA and 23S rRNA for distinguishing Listeria species, the complete 23S rRNA sequences for all Listeria species were determined by using the type strains. We designed and experimentally validated a universal 23S rRNA sequencing method, which included PCR amplification of the rDNA gene and direct cycle sequencing of the amplicon with eubacterial p...